Reacción a "Mitochondrial DNA mutations in oocytes do not increase with age"

This new study brings important news about the DNA of the cell's "powerhouses",  the mitochondria, and how it behaves in women’s eggs and how it relates to age. This research is particularly relevant in today’s european demographic context and especially in Spain, where a growing trend towards late motherhood is observed, together with the increasingly widespread use of assisted reproductive technologies worldwide. The good news is that, unlike what happens in other tissues of the body such as blood or saliva, where mitochondrial DNA (mtDNA) mutations increase with age, human oocytes do not accumulate more mutations as women age, at least between 20 and 42 years of age. This suggests that mtDNA in oocytes is protected against aging and its potential negative impact on cellular function. The researchers believe this is due to a process called “purifying selection,” a kind of quality control that eliminates problematic variants in eggs.

The study also adds on the understanding of a key concept in mitochondrial biology: the mitochondrial “bottleneck” in the germline. In this process, from the thousands of mtDNA copies that a mother carries, only a very small number are passed on to her eggs, as if everything is being filtered through a funnel. This process is fundamental for mtDNA inheritance. Researchers estimated that the size of this “funnel” is about 909 mtDNA units for all heritable mutations. However, for the more common mutations, the bottleneck is much tighter, indicating that common mutations are subject to a different kind of selective pressure. Another reassuring detail is that the size of this “bottleneck” does not change significantly with the mother’s age. All of this has been made possible thanks to an ultra-high-precision sequencing technology called “duplex sequencing”, and confirms previos findings on the bottleneck dynamics performed with lower-accuracy technique.

Overall, this study is reassuring for people trying to conceive childern at later ages, because, although chromosomal abnormalities increase with maternal age, at least they should not expect a higher level of mutations in their mtDNA due to their advanced age. However, it is important to consider the limitations when generalizing these findings: the study was based on a relatively small sample of 22 women, and only 8 in the older group, with a total of 80 oocytes analyzed, which limits the statistical power to detect subtle increases. Importantly, all participants were undergoing in vitro fertilization (IVF) treatments with controlled ovarian stimulation, and the oocytes used for the study were predominantly immature. This specific population and the hormonal stimulation process, as also acknowldeged by the authors, calls for future studies with larger and more diverse samples needed to confirm and expand upon these reassuring findings.